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105004，試管旋轉混勻器
基本售價：1428.00元
下單前請聯系客服詢問貨期，單位：箱試管旋轉混勻器是一款設計獨特的產品， 旋轉速度溫和，通過對試管板的調節，使樣品處于任意角度旋轉，廣泛應用于分子生物學、化學、臨床應用等各種需求混合的場合。可同時安裝不同的試管板，并可調整不同的角度，以提高實驗效率。? 體積小，操作簡便；? 標配五種試管板，滿足客戶多種需求；? 開啟狀態下，輕按試管板，可反方向旋轉；? 噪音低，運轉平穩；? 高性能馬達，使用壽命長。
105003，迷你漩渦混勻器
基本售價：686.00元
下單前請聯系客服詢問貨期，單位：箱 迷你漩渦混勻器是專注于點動功能的試管振蕩器，緊湊小巧的設計，觸發式啟動開關，安全便捷，是實驗室必不可少的工具之一，可用于振蕩試管、離心管，混合效果顯著。? 結構小巧緊湊， 質量可靠；? 專用于振蕩直徑小于30mm的容器；? 工作墊片為耐磨材料制成，使用壽命長；? 精制鋼材底座，運行穩定。
中科都菱-86/-150℃超低溫保存箱系列 MDF-86V838E
基本售價：0.00元
高性價比自主研發的高效混合制冷劑配方,節能50%人性化設計采用新型門把手，可附加掛鎖；配備測試孔設計；可調節的不銹鋼擱架；內門可拆卸，便于客戶清潔與除霜；報警方式箱內溫度高低溫報警，聲光報警，可選配短信通知等報警方式；
中科都菱-86/-150℃超低溫保存箱系列 MDF-86V688
基本售價：0.00元
特殊聚氨酯發泡保溫材料-86系列產品內外溫差可達110℃，特殊聚氨酯發泡保溫層避免破裂及變形，150mm的保溫材料厚度可確保內部溫度的穩定性制冷系統雙機復疊制冷系統，碳氫制冷劑節能50%特殊降噪結構每個可能產生噪音的部件都裝有特殊材質的減震材料，機組周圍裝有高密度防火吸音棉
中科都菱-86/-150℃超低溫保存箱系列 MDF-86V588E
基本售價：0.00元
產品特點自主研發的高效混合制冷劑配方,節能50%安全保障在恢復供電時，所有設備的同時啟動會對電網造成較大沖擊，從而可能導致斷路器跳閘；針對這種情況特別設計的設備延時啟動功能可使設備；人性化設計內門可拆卸，便于客戶除霜與清潔；
中科都菱-86/-150℃超低溫保存箱系列 MDF-86V408E
基本售價：0.00元
高性價比自主研發的高效混合制冷劑配方,節能50%人性化設計采用新型門把手，可附加掛鎖；配備測試孔設計；可調節的不銹鋼擱架；內門可拆卸，便于客戶清潔與除霜；報警方式具備高低溫報警、傳感器故障報警、溫控器故障報警、斷電報警、門開報警、環溫報警、冷凝器故障報警、過濾網檢查報警、電壓異常報警、電池電量低報警。
中科都菱-86/-150℃超低溫保存箱系列 MDF-86V340E
基本售價：0.00元
高性價比自主研發的高效混合制冷劑配方,節能50%降溫速度更快降溫速度提升20%，均勻性提高30%單一制冷自復疊一個制冷回路，相比普通雙級復疊系統減少一倍的系統部件
中科都菱-86/-150℃超低溫保存箱系列 MDF-86V188E
基本售價：0.00元
高性價比自主研發的混合工質可使產品節能50%標配功能標配測試孔，USB接口以及遠程報警接口報警方式箱內溫度高低溫報警，聲光報警，可選配短信通知等報警方式；
中科都菱-86/-150℃超低溫保存箱系列 MDF-86V50
基本售價：0.00元
采用優質冷軋鋼板內壁為優質304不銹鋼鋼板，經久耐用，安全可靠高精度電腦控制系統精準保持箱體內部溫度精確穩定，箱內溫度-40~-86℃可調采用單級自復疊制冷系統制冷速度快，耗電量低，制冷量大
中科都菱-86/-150℃超低溫保存箱系列 MDF-86H485
基本售價：0.00元
溫控方式采用國際著名名牌高精度電腦溫控系統，精準保持箱體內部溫度精確穩定，箱內溫度-40~-86℃可調；制冷系統采用優化的單級自復疊制冷系統設計，制冷速度快，耗電量低，制冷量大；安全保障具備高低溫報警，傳感器故障報警功能；具備聲音蜂鳴和燈光閃爍報警方式；
中科都菱-86/-150℃超低溫保存箱系列 MDF-86H105
基本售價：0.00元
箱體箱體外殼采用冷板噴涂；內膽為優質304不銹鋼，經久耐用，安全可靠；溫控方式采用全銅管蒸發器，精準的電子溫度控制及顯示，精度達到0.1℃；特色功能安全門鎖設計；大屏幕數字顯示便于觀察；標配的測試孔；
METHER高端系列-86/-150℃超低溫保存箱 MDF-86V588D（雙系統）
基本售價：0.00元
雙重保障采用兩套獨立的制冷系統，其中一套故障時，另一套可維持-70的柜內溫度快速降溫雙系統同時工作能實現快速降溫，降溫時間縮短50%以上智能控溫觸摸屏，溫度曲線、報警信息記錄及查詢，用戶管理模式，自我診斷
METHER高端系列-86/-150℃超低溫保存箱 MDF-86V728
基本售價：0.00元
特殊降噪音結構每個可能產生噪音的部件都裝有特殊材質的減震材料，機組周圍裝有高密度防火吸音棉溫度控制微電腦控制，箱內溫度-40~-86℃可調，超溫報警，斷電記憶，調節精度為0.1℃；制冷系統優化的雙機復疊式制冷系統，可以實現最高35%的節能效果
METHER高端系列-86/-150℃超低溫保存箱 MDF-86V838
基本售價：0.00元
特殊降噪音結構每個可能產生噪音的部件都裝有特殊材質的減震材料，機組周圍裝有高密度防火吸音棉溫度控制微電腦控制，箱內溫度-60~-86℃可調，超溫報警，斷電記憶，調節精度為0.1℃；制冷系統優化的雙機復疊式制冷系統，可以實現最高35%的節能效果
METHER高端系列-86/-150℃超低溫保存箱 MDF-86V588
基本售價：0.00元
制冷劑采用自主研發的無氟碳氫環保制冷劑VIPIIPLUS先進技術VIPIIPLUS第二代真空絕熱板技術，使冰箱有良好的保溫性能，專為進入電梯而設計產品特點專為生物樣本庫優化的產品結構，可以提供最大4萬份樣本的存儲空間雙機復疊技術，傳統技術穩定性安全可靠，C/H制冷劑綠色無污染，更節能，第二代VIP技術，保溫性能好，減小占地面積，樣本儲存量最優
METHER高端系列-86/-150℃超低溫保存箱 MDF-86V338
基本售價：0.00元
顯示面板屏幕尺寸為7英寸液晶觸摸屏，可直觀的進行操作及顯示各種與設備相關的信息；報警系統具備高低溫報警、傳感器故障報警、冷凝器臟堵報警、環溫異常報警、電壓異常報警、斷電報警、門開報警、電池電量低報警、遠程報警接口，所有的報警信息以及歷史記錄可在液晶屏查詢。USB數據存儲功能具備USB數據存儲功能，可存儲10年以上的數據，方便查詢歷史記錄；雙機復疊技術，耐43℃環境溫度，超保溫
METHER高端系列-86/-150℃超低溫保存箱 MDF-86V408
基本售價：0.00元
控溫方式觸控屏溫度控制系統，控制更精確，溫度數字與曲線雙顯示。溫度-10~-86℃自由調節，滿足不同物品不同溫區存放，采用優質工業設備專用電阻式7英寸觸控屏，防塵、防水汽雙測試孔/溫度打印標配雙測試孔，USB接口以及遠程報警接口，USB具有數據導出功能，配備熱敏溫度記錄打印機，數據瀏覽功能可隨時掌握運行周期狀況：完善的聲光報警功能高溫、低溫、開門、過濾網堵塞、系統故障、傳感器故障、環溫高報警等報警功能，確保箱內物品存放安全；密碼保護功能系統，防止隨意調整運行參數；雙機復疊技術，傳統技術穩定性安全可靠，C/H制冷劑綠色無污染，更節能，第二代VIP技術，保溫性能好，減小占地面積，樣本儲存量最優
METHER高端系列-86/-150℃超低溫保存箱 MDF-86V838D（雙系統）
基本售價：0.00元
雙重保障采用兩套獨立的制冷系統，其中一套故障時，另一套可維持-70℃的柜內溫度快速降溫雙系統同時工作能實現快速降溫，降溫時間縮短50%以上智能控溫觸摸屏，溫度曲線、報警信息記錄及查詢，用戶管理模式，自我診斷
一氧化氮合成酶-2（誘導型）抗體 | RabbitAnti-iNOS antibody
基本售價：1098.00元
產品編號bs-0162R英文名稱iNOS中文名稱一氧化氮合成酶-2（誘導型）抗體別 名i NOS; Nitric Oxide Synthase, Inducible; HEP NOS; Hepatocyte NOS; HEPNOS; Inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; INOS; Inosl; MAC NOS; Macrophage NOS; Nitric oxide synthase 2 inducible macrophage; Nitric oxide synthase 2A (inducible hepatocytes); Nitric oxide synthase inducible; NOS 2; NOS 2A; NOS; Nos II; NOS type II; Nos2; NOS2A; NOS2_HUMAN. Specific References (47) | bs-0162R has been referenced in 47 publications.111 [IF=2.35] Li, Guofu, et al. "The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats." International Journal of Molecular Medicine 38.3 (2016): 767-775. WB ; Rat. 222111 [IF=4.56] Shanshan et al. Phospholipase A2 of Peroxiredoxin 6 Plays a Critical Role in Cerebral Ischemia/Reperfusion Inflammatory Injury. (2017) Front.Cell.Neurosc. 11:99 WB ; Rat. 222PubMed:28424593 111 [IF=2.24] Zhang et al. High altitude increases the expression of hypoxia-inducible factor 1α and inducible nitric oxide synthase with intest-inal mucosal barrier failure in rats. (2015) Int.J.Clin.Exp.Patho. 8:5189-95 IHC ; Rat. 222PubMed:26191216 111 [IF=5.64] Wang et al. CXCL1 derived from tumor-associated macrophages promotes breast cancer metastasis via activating NF-κB/SOX4 signaling. (2018) Cell.Death.Dis. 9:880 WB ; Mouse. 222PubMed:30158589 111 [IF=2.8] Liu J et al. Alterations of antioxidant indexes and inflammatory cytokine expression aggravated hepatocellular apoptosis through mitochondrial and death receptor-dependent pathways in Gallus gallus exposed to arsenic and copper. Environ Sci Pollut Res Int WB ; Chicken. 222PubMed:29569195 111 [IF=2.332] Zhao X et al. Total flavones of fermentation broth by co-culture of Coprinus comatus and Morchella esculenta induces an anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages cells via the MAPK signaling pathway.(2018) Microb Pathog.125:431-437. WB ; Mouse. 222PubMed:30316005 111 [IF=2.655] Horváth E et al. Ischemic damage and early inflammatory infiltration are different in the core and penumbra lesions of rat brain after transient focal cerebral ischemia. J Neuroimmunol. 2018 Nov 15;324:35-42. IHC-P ; Rat. 222PubMed:30212790 111 [IF=4.183] Imoto K et al. Periostin Mediates Right Ventricular Failure through Induction of Inducible Nitric Oxide SynthaseExpression in Right Ventricular Fibroblasts from Monocrotaline-Induced Pulmonary Arterial Hypertensive Rats. Int J Mol Sci. 2018 Dec 24;20(1). IHC-P ; Rat. 222PubMed:30586863 111 [IF=3.118] Lv R et al. Polydatin alleviates traumatic spinal cord injury by reducing microglial inflammation via regulation of iNOS and NLRP3 inflammasome pathway. Int Immunopharmacol. 2019 Feb 18;70:28-36. IHC-P ; Rat. 222PubMed:30785088 111 [IF=2.535] Lei J et al. LncRNA SNHG1 Alleviates IL-1β-induced Osteoarthritis by Inhibiting miR-16-5p-mediated p38 MAPK and NF-κB Signaling Pathways. Biosci Rep. 2019 Aug 5. pii: BSR20191523. WB ; Human. 222PubMed:31383786 111 [IF=2.914] Guo MH et al. Elemental imbalance elicited by arsenic and copper exposure lead to oxidative stress and immunotoxicity in chicken gizzard, activating the protective effects of heat shock proteins. Environmental Science and Pollution Research. 2019. WB ; Chicken. 222111 [IF=3.87] Jiao F et al. AGK2 Alleviates Lipopolysaccharide Induced Neuroinflammation through Regulation of Mitogen-Activated Protein Kinase Phosphatase-1. J Neuroimmune Pharmacol. 2019 Nov 30. IHF ; Mouse. 222PubMed:31786712 111 [IF=3.483] Huang P et al. Theoretical basis of nitrosomyoglobin formation in a dry sausage model by coagulase-negative staphylococci: Behavior and expression of nitric oxide synthase. Meat Sci. 2019 Nov 27;161:108022. WB ; coagulase-negative staphylococci (CNS) and P. pentosaceus. 222PubMed:31838366 111 [IF=3.58] El-Lakany MA et al. Ovariectomy provokes inflammatory and cardiovascular effects of endotoxemia in rats: Dissimilar benefits of hormonal supplements. Toxicol Appl Pharmacol. 2020 Feb 21;393:114928. IHC-P ; Rat. 222PubMed:32092384 111 [IF=3.361] Wang M et al. Berberine combined with cyclosporine A alleviates acute graft-versus-host disease in murine models. Int Immunopharmacol. 2020 Feb 9;81:106205. IHC-P ; Mouse. 222PubMed:32050154 111 [IF=3.761] Toru Nishikawa et al. Therapeutic potential for insulin on type 1 diabetes‐associated periodontitis: analysis of experimental periodontitis in streptozotocin‐induced diabetic rats. J Diabetes Investig . 2020 Apr 17. IHC ; rat. 222PubMed:32302049 111 [IF=8.352] Ye Hea et al. Improved osteointegration by SEW2871-encapsulated multilayers on micro-structured titanium via macrophages recruitment and immunomodulation. Applied Materials Today 20 (2020) 100673 IHC/IF ; Rat/Mouse. 222111 [IF=2.5] Yang F et al. The effects of binge alcohol exposure on tooth movement and associated root resorption in rats. Alcohol . 2020 Nov;88:1-9. IHC-P ; Rat. 222PubMed:32574660 111 [IF=4.225] Jiang Q et al. Particulate Matter 2.5 Induced Developmental Cardiotoxicity in Chicken Embryo and Hatchling. Front Pharmacol. 2020 Jun 5;11:841. WB ; Chicken. 222PubMed:32581800 111 [IF=] WB ; mouse. 222111 [IF=7.919] Chen Y et al. A selected small molecule prevents inflammatory osteolysis through restraining osteoclastogenesis by modulating PTEN activityClin Transl Med.2020 Dec;10(8):e240. WB、IHC ; Mouse. 222PubMed:33377656 111 [IF=7.727] Xue Wang. et al. Engineered liposomes targeting the gut–CNS Axis for comprehensive therapy of spinal cord injury. J Control Release. 2021 Mar;331:390 WB,IHC ; Mouse. 222111 [IF=3.909] Qian Xueshen. et al. Periodontitis Deteriorates Cognitive Function and Impairs Neurons and Glia in a Mouse Model of Alzheimer’s Disease. J Alzheimers Dis. 2021 Jan;79(4):1785-1800 WB ; Mouse. 222111 [IF=3.69] Xiaoyan Xia. et al. Ameliorative effect of white tea from 50-year-old tree of Camellia sinensis L. (Theaceae) on kidney damage in diabetic mice via SIRT1/AMPK pathway. J Ethnopharmacol. 2021 May;272:113919 WB ; Mouse. 222111 [IF=8.758] Yinying Sheng. et al. Development and In Vitro Biodegradation of Biomimetic Zwitterionic Phosphorylcholine Chitosan Coating on Zn1Mg Alloy. Acs Appl Mater Inter. 2020;12(49):54445–54458 IF ; Mouse. 222111 [IF=3.368] Liu Tang. et al. Topical Application with Conjugated Linoleic Acid Ameliorates 2, 4‐dinitrofluorobenzene‐Induced Atopic Dermatitis‐like Lesions in BALB/c Mice. Exp Dermatol. 2021 Feb;30(2):237-248 WB ; Mouse. 222111 [IF=2.965] Sawada Noritaka. et al. Glucagon-Like Peptide-1 Receptor Agonist Liraglutide Ameliorates the Development of Periodontitis. J Diabetes Res. 2020;2020:8843310 IHC ; Rat. 222111 [IF=5.88] Shuai Chen. et al. Chondroitin sulfate modified 3D porous electrospun nanofiber scaffolds promote cartilage regeneration. Mat Sci Eng C-Mater. 2021 Jan;118:111312 IHC ; Rabbit. 222111 [IF=8.724] Penghui Zhang. et al. Effect of cyclic mechanical loading on immunoinflammatory microenvironment in biofabricating hydroxyapatite scaffold for bone regeneration. Bioact Mater. 2021 Oct;6:3097 WB ; Mouse. 222111 [IF=3.943] Kai Kang. et al. FGF21 alleviates chronic inflammatory injury in the aging process through modulating polarization of macrophages. Int Immunopharmacol. 2021 Jul;96:107634 WB ; Mouse. 222研究領域細胞生物 免疫學 神經生物學 信號轉導 轉錄調節因子 激酶和磷酸酶 抗體來源Rabbit克隆類型Polyclonal交叉反應Human, Mouse, Rat, 產品應用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.理論分子量130kDa細胞定位細胞核 細胞漿 性 狀Liquid濃 度1mg/ml免 疫 原KLH conjugated synthetic peptide derived from human NOS-2: 1051-1144/1144 亞 型IgG純化方法affinity purified by Protein A緩 沖 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.注意事項This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.PubMedPubMed 產品介紹Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. Nitric oxide synthase is expressed in liver, macrophages, hepatocytes, synoviocytes, stimulated glial cells and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).Function:Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.Subunit:Homodimer. Binds SLC9A3R1.Tissue Specificity:Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.Similarity:Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.SWISS:P35228Gene ID:4843Database links:Entrez Gene: 4843 HumanEntrez Gene: 18126 MouseEntrez Gene: 24599 RatOmim: 163730 HumanSwissProt: P35228 HumanSwissProt: P29477 MouseSwissProt: Q06518 RatUnigene: 709191 HumanUnigene: 2893 MouseUnigene: 10400 Rat合成與降解（Synthesis and Degradation）催化生物體內一氧化氮（NO）生成的酶。分神經型一氧化氮合成的酶（nNOS or NOS-1）、誘導型一氧化氮合成的酶（iNOS or NOS-2）、內皮型一氧化氮合成的酶（eNOS or NOS-3）。產品圖片Sample:U251 Cell Lysate at 40 ugPrimary: Anti-iNOS(bs-0162R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 130kDObserved band size: 130kDSample:Lane 1: Mouse Raw264.7 tissue lysatesLane 2: Human Jurkat cell lysatesPrimary: Anti-iNOS (bs-0162R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 130 kDaObserved band size: 140 kDaParaformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (inos) Polyclonal Antibody, Unconjugated (bs-0162R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (iNOS) Polyclonal Antibody, Unconjugated (bs-0162R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-iNOS Polyclonal Antibody, Unconjugated(bs-0162R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-iNOS Polyclonal Antibody, Unconjugated(bs-0162R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
缺氧誘導因子1α /低氧誘導因子-1抗體 | RabbitAnti-HIF-1 Alpha antibody
基本售價：1098.00元
產品編號bs-0737R英文名稱HIF-1 Alpha中文名稱缺氧誘導因子1α /低氧誘導因子-1抗體別 名ARNT interacting protein; ARNT-interacting protein; Basic helix loop helix PAS protein MOP1; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF 1alpha; HIF-1-alpha; HIF1 A; HIF1 Alpha; HIF1; HIF1-alpha; HIF1A; HIF1A_HUMAN; Hypoxia inducible factor 1 alpha; Hypoxia inducible factor 1 alpha isoform I.3; Hypoxia inducible factor 1 alpha subunit; Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor; Hypoxia inducible factor 1, alpha subunit (basic helix loop helix transcription factor); Hypoxia inducible factor1alpha; Hypoxia-inducible factor 1-alpha; Hypoxia-inducible factor-1a; Member of PAS protein 1; Member of PAS superfamily 1; Member of the PAS Superfamily 1; MOP 1; MOP1; PAS domain-containing protein 8; PASD 8; PASD8. Specific References (22) | bs-0737R has been referenced in 22 publications.[IF=5.595] Liu T et al. MicroRNA-493 targets STMN-1 and promotes hypoxia-induced epithelial cell cycle arrest in G/M and renal fibrosis. (2018) FASEB J. Sep 05 WB ; Mouse. PubMed:30183377 [IF=5.08] Zhang, Huimin, et al. "Vascular Normalization Induced by Sinomenine Hydrochloride Results in Suppressed Mammary Tumor Growth and Metastasis." Scientific Reports 5 (2015). IHC-P ; Mouse. PubMed:25749075 [IF=4.91] Fan, Shengjun, et al. "Opposite angiogenic outcome of curcumin against ischemia and Lewis lung cancer models:in silico, in vitro and in vivo studies." Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease (2014). WB ; Mouse. PubMed:24970744 [IF=4.556] Yang X et al. Synthesis and bioevaluation of novel 18FDG-conjugated 2-nitroimidazole derivatives for tumor-hypoxia imaging. Mol Pharm. 2019 May 6;16(5):2118-2128. IHC&IF ; Mouse&Human. PubMed:30964298 [IF=4.539] Talwar H et al. MEK2 Negatively Regulates Lipopolysaccharide-Mediated IL-1β Production through HIF-1α Expression. J Immunol. 2019 Mar 15;202(6):1815-1825. WB&IP ; Mouse. PubMed:30710049 [IF=4.44] Madka, Venkateshwar, et al. "Targeting mTOR and p53 signaling inhibits muscle invasive bladder cancer in vivo." Cancer Prevention Research 9.1 (2016): 53-62. IHC-P ; Mouse. PubMed:26577454 [IF=4.19] Talwar, Harvinder, et al. "MKP-1 negatively regulates LPS-mediated IL-1β production through p38 activation and HIF-1α expression." Cellular Signalling (2017). Mouse. PubMed:28238855 [IF=3.743] Wang D et al. Effects of hypoxia and ASIC3 on nucleus pulposus cells: From cell behavior to molecularmechanism. Biomed Pharmacother. 2019 Jun 12;117:109061. WB ; Rabbit. PubMed:31202172 [IF=3.53] Woolf, Eric C., et al. "The Ketogenic Diet Alters the Hypoxic Response and Affects Expression of Proteins Associated with Angiogenesis, Invasive Potential and Vascular Permeability in a Mouse Glioma Model." PLOS ONE10.6 (2015): e0130357. WB ; Mouse. PubMed:26083629 [IF=3.288] Chai D et al. β2-microglobulin has a different regulatory molecular mechanism between ER+ and ER- breast cancer with HER2.BMC Cancer. 2019 Mar 12;19(1):223. IHC-P ; Human. PubMed:30866857 [IF=2.989] Ju X et al. Catalpol Promotes the Survival and VEGF Secretion of Bone Marrow-Derived Stem Cells and Their Role in Myocardial Repair After Myocardial Infarction in Rats.Cardiovasc Toxicol. 2018 May 11. WB ; Rat. PubMed:29752623 [IF=2.91] Shou, Zhu, et al. "Expression and prognosis of FOXO3a and HIF-1?? in nasopharyngeal carcinoma."Journal of cancer research and clinical oncology 138.4 (2012): 585-593.. WB ; Human. PubMed:22209974 [IF=2.784] Yang D et al. Normobaric oxygen inhibits AQP4 and NHE1 expression in experimental focal ischemic stroke. (2018) Int. J. Mol. Med. WB ; Rat . PubMed:30592266 [IF=2.766] Dao DT et al. A paradoxical method to enhance compensatory lung growth: Utilizing a VEGF inhibitor.(2018) PLoS One. WB ; Mouse. PubMed:30566445 [IF=2.566] Yang Z et al. Tenascin-C is involved in promotion of cancer stemness via the Akt/HIF1ɑ axis in esophageal squamous cell carcinoma.Exp Mol Pathol. 2019 Mar 20. WB ; Human. PubMed:30904401 [IF=2.49] Yang, Ya, et al. "Expression of RAP1B is associated with poor prognosis and promotes an aggressive phenotype in gastric cancer." Oncology reports 34.5 (2015): 2385-2394. IHC-P ; Human. PubMed:26329876 [IF=1.64] Guo, Wei, et al. "Transplantation of endothelial progenitor cells in treating rats with IgA nephropathy." BMC Nephrology 15.1 (2014): 110. WB ; Rat. PubMed:25012471 [IF=1.55] Yang, Jinjiang, Ying Lu, and Ai Guo. "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." Molecular Medicine Reports 14.5 (2016): 4075-4082. WB ; Rat. PubMed:27665780 [IF=1.41] Song et al. Effects of HSYA on the proliferation and apoptosis of MSCs exposed to hypoxic and serum deprivation conditions. (2018) Exp.Ther.Med. 15:5251-5260 WB ; Rat. PubMed:29904409 [IF=.5] Talwar, Harvinder, et al. "The dataset describes: HIF-1 α expression and LPS mediated cytokine production in MKP-1 deficient bone marrow derived murine macrophages." Data in Brief (2017). WB ; Mouse. PubMed:28765831 [IF=0] Talwar et al. The dataset describes: HIF-1 α expression and LPS mediated cytokine production in MKP-1 deficient bone marrow derived murine macrophages. (2017) Data.Brie. 14:56-61 WB ; Mouse. PubMed:28765831 [IF=3.414] Li ZH et al. You-Gui-Yin improved the reproductive dysfunction of male rats with chronic kidney disease via regulating the HIF1α-STAT5 pathway. J Ethnopharmacol. 2020 Jan 10;246:112240. WB ; Rat. PubMed:31526861 研究領域腫瘤 細胞生物 神經生物學 信號轉導 細胞凋亡 抗體來源Rabbit克隆類型Polyclonal交叉反應Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, 產品應用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （石蠟切片需做抗原修復）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.分 子 量92kDa細胞定位細胞核 細胞漿 性 狀Liquid濃 度1mg/ml免 疫 原KLH conjugated synthetic peptide derived from middle of human HIF-1 Alpha:341-450/826 亞 型IgG純化方法affinity purified by Protein A儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.PubMedPubMed 產品介紹Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator(ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.Function:Functions as a master transcriptional regulator of theadaptive response to hypoxia. Under hypoxic conditions activatesthe transcription of over 40 genes, including, erythropoietin,glucose transporters, glycolytic enzymes, vascular endothelialgrowth factor, and other genes whose protein products increaseoxygen delivery or facilitate metabolic adaptation to hypoxia.Plays an essential role in embryonic vascularization, tumorangiogenesis and pathophysiology of ischemic disease. Binds to coreDNA sequence 5'-[AG]CGTG-3' within the hypoxia response element(HRE) of target gene promoters. Activation requires recruitment oftranscriptional coactivators such as CREBPB and EP300. Activity isenhanced by interaction with both, NCOA1 or NCOA2. Interaction withredox regulatory protein APEX seems to activate CTAD andpotentiates activation by NCOA1 and CREBBP.Subunit:Interacts with COPS5 subunit of COP9 signalosome complex,leading to the regulation of its stability. Interacts with TSGA10(By similarity). Efficient DNA binding requires heterodimerizationof an alpha and a beta/ARNT subunit. Binds to the TAZ-type 1domains of CREBBP and EP300. Interacts with NCOA1, NCOA2, APEX andHSP90. Interacts with VHL which docks HFA1 to the E3 ubiquitinligase complex for subsequent destruction. Interaction, via the ODDdomain, with the beta domain of VHLL, protects HIF1A fromdestruction by competing against the destructive targetinginitiated by VHL.Subcellular Location:Cytoplasm. Nucleus.Tissue Specificity:Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors.Post-translational modifications:In normoxia, is hydroxylated on Pro-402 and Pro-564 in theoxygen-dependent degradation domain (ODD) by EGLN1/PHD1 andEGLN2/PHD2. EGLN3/PHD3 has also been shown to hydroxylate Pro-564.The hydroxylated prolines promote interaction with VHL, initiatingrapid ubiquitination and subsequent proteasomal degradation. Underhypoxia, proline hydroxylation is impaired and ubiquitination isattenuated, resulting in stabilization.In normoxia, is hydroxylated on Asn-803 by HIF1AN, thusabrogating interaction with CREBBP and EP300 and preventingtranscriptional activation.S-nitrosylation of Cys-800 may be responsible for increasedrecruitment of p300 coactivator necessary for transcriptionalactivity of HIF-1 complex.Acetylation of Lys-532 by ARD1 increases interaction with VHLand stimulates subsequent proteasomal degradation.Requires phosphorylation for DNA-binding.Similarity:Contains 1 basic helix-loop-helix (bHLH) domain.Contains 1 PAC (PAS-associated C-terminal) domain.Contains 2 PAS (PER-ARNT-SIM) domains.SWISS:Q16665Gene ID:3091Database links:Entrez Gene: 3091 HumanEntrez Gene: 15251 MouseOmim: 603348 HumanSwissProt: Q16665 HumanSwissProt: Q61221 MouseUnigene: 597216 HumanUnigene: 3879 MouseUnigene: 446610 MouseImportant Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.缺氧誘導因子1α不僅對于機體在缺氧條件下維持正常的生理功能具有特別重要的意義,并在腫瘤的生長以及神經細胞凋亡等病理過程中起重要作用. HIF1 alpha能調節許多下游基因的表達水平.哺乳動物細胞在低氧壓力條件下出現HIF。HIF是一種轉錄因子，對細胞的缺氧起穩定作用。產品圖片Sample:Lane 1: Hela (Human) Cell Lysate at 30 ugLane 2: A431 (Human) Cell Lysate at 30 ugLane 3: U251 (Human) Cell Lysate at 30 ugLane 4: HepG2 (Human) Cell Lysate at 30 ugPrimary: Anti-HIF-1 Alpha (bs-0737R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 120 kDObserved band size: 120 kDParaformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HIF-1 Alpha) Polyclonal Antibody, Unconjugated (bs-0737R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HIF-1 Alpha) Polyclonal Antibody, Unconjugated (bs-0737R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-HIF-1-Alpha Polyclonal Antibody, Unconjugated(bs-0737R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: rat lung tissue(Smoking); 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-HIF-1-Alpha Polyclonal Antibody, Unconjugated(bs-0737R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingHela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HIF-1 Alpha) polyclonal Antibody, Unconjugated (bs-0737R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Blank control (blue line): Hela (blue).Primary Antibody (green line): Rabbit Anti- HIF-1 Alpha antibody (bs-0737R)Dilution: 1μg /10^6 cells;Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITCDilution: 1μg /test.ProtocolThe cells were fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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